Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun)

Numlil Khaira Rusdi; Aditya Inggrayni; Adrian Muhamad Rizky; Erni Hernawati Purwaningsih; Andon Hestiantoro; Berna Elya; Kusmardi

doi:10.1063/5.0102969

Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun)

Numlil Khaira Rusdi, Aditya Inggrayni, Adrian Muhamad Rizky, Erni Hernawati Purwaningsih, Andon Hestiantoro, Berna Elya, Kusmardi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.

Original language	English
Title of host publication	6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021
Editors	Siti Fauziyah Rahman, Ahmad Zakiyuddin, Yudan Whulanza, Nurul Intan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735443716
DOIs	https://doi.org/10.1063/5.0102969
Publication status	Published - 16 Aug 2022
Event	6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 - Depok, Virtual, Indonesia Duration: 7 Jul 2021 → 8 Jul 2021

Publication series

Name	AIP Conference Proceedings
Volume	2537
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Country/Territory	Indonesia
City	Depok, Virtual
Period	7/07/21 → 8/07/21

Keywords

acute toxicity
kidney
LD50
liver
soybean

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/5.0102969

Cite this

Rusdi, N. K., Inggrayni, A., Rizky, A. M., Purwaningsih, E. H., Hestiantoro, A., Elya, B., & Kusmardi (2022). Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun). In S. F. Rahman, A. Zakiyuddin, Y. Whulanza, & N. Intan (Eds.), 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021 Article 040009 (AIP Conference Proceedings; Vol. 2537). American Institute of Physics Inc.. https://doi.org/10.1063/5.0102969

Rusdi, Numlil Khaira ; Inggrayni, Aditya ; Rizky, Adrian Muhamad et al. / Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun). 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. editor / Siti Fauziyah Rahman ; Ahmad Zakiyuddin ; Yudan Whulanza ; Nurul Intan. American Institute of Physics Inc., 2022. (AIP Conference Proceedings).

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abstract = "ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.",

keywords = "acute toxicity, kidney, LD50, liver, soybean",

author = "Rusdi, {Numlil Khaira} and Aditya Inggrayni and Rizky, {Adrian Muhamad} and Purwaningsih, {Erni Hernawati} and Andon Hestiantoro and Berna Elya and Kusmardi",

note = "Funding Information: Thanks to the Ministry of Education, Culture, Research, and Technology (PDD contract number 304, 2021); and the Research and Development of Universitas Muhammadiyah Prof. DR. HAMKA, for their valuable support. Publisher Copyright: {\textcopyright} 2022 American Institute of Physics Inc.. All rights reserved.; 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 ; Conference date: 07-07-2021 Through 08-07-2021",

year = "2022",

month = aug,

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doi = "10.1063/5.0102969",

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series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Rahman, {Siti Fauziyah} and Ahmad Zakiyuddin and Yudan Whulanza and Nurul Intan",

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Rusdi, NK, Inggrayni, A, Rizky, AM, Purwaningsih, EH , Hestiantoro, A , Elya, B & Kusmardi 2022, Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun). in SF Rahman, A Zakiyuddin, Y Whulanza & N Intan (eds), 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021., 040009, AIP Conference Proceedings, vol. 2537, American Institute of Physics Inc., 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021, Depok, Virtual, Indonesia, 7/07/21. https://doi.org/10.1063/5.0102969

Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun). / Rusdi, Numlil Khaira; Inggrayni, Aditya; Rizky, Adrian Muhamad et al.
6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. ed. / Siti Fauziyah Rahman; Ahmad Zakiyuddin; Yudan Whulanza; Nurul Intan. American Institute of Physics Inc., 2022. 040009 (AIP Conference Proceedings; Vol. 2537).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun)

AU - Rusdi, Numlil Khaira

AU - Inggrayni, Aditya

AU - Rizky, Adrian Muhamad

AU - Purwaningsih, Erni Hernawati

AU - Hestiantoro, Andon

AU - Elya, Berna

AU - Kusmardi,

N1 - Funding Information: Thanks to the Ministry of Education, Culture, Research, and Technology (PDD contract number 304, 2021); and the Research and Development of Universitas Muhammadiyah Prof. DR. HAMKA, for their valuable support. Publisher Copyright: © 2022 American Institute of Physics Inc.. All rights reserved.

PY - 2022/8/16

Y1 - 2022/8/16

N2 - ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.

AB - ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.

KW - acute toxicity

KW - kidney

KW - LD50

KW - liver

KW - soybean

UR - http://www.scopus.com/inward/record.url?scp=85138222659&partnerID=8YFLogxK

U2 - 10.1063/5.0102969

DO - 10.1063/5.0102969

M3 - Conference contribution

AN - SCOPUS:85138222659

T3 - AIP Conference Proceedings

BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices

A2 - Rahman, Siti Fauziyah

A2 - Zakiyuddin, Ahmad

A2 - Whulanza, Yudan

A2 - Intan, Nurul

PB - American Institute of Physics Inc.

T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021

Y2 - 7 July 2021 through 8 July 2021

ER -

Rusdi NK, Inggrayni A, Rizky AM, Purwaningsih EH , Hestiantoro A , Elya B et al. Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun). In Rahman SF, Zakiyuddin A, Whulanza Y, Intan N, editors, 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021. American Institute of Physics Inc. 2022. 040009. (AIP Conference Proceedings). doi: 10.1063/5.0102969

Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun)

Abstract

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Keywords

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