TY - GEN
T1 - Acute Toxicity of Soybean Extract with Targeted Lunasin (ET-Lun)
AU - Rusdi, Numlil Khaira
AU - Inggrayni, Aditya
AU - Rizky, Adrian Muhamad
AU - Purwaningsih, Erni Hernawati
AU - Hestiantoro, Andon
AU - Elya, Berna
AU - Kusmardi,
N1 - Funding Information:
Thanks to the Ministry of Education, Culture, Research, and Technology (PDD contract number 304, 2021); and the Research and Development of Universitas Muhammadiyah Prof. DR. HAMKA, for their valuable support.
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.
AB - ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7, 4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n = 10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n=5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically nontoxic.
KW - acute toxicity
KW - kidney
KW - LD50
KW - liver
KW - soybean
UR - http://www.scopus.com/inward/record.url?scp=85138222659&partnerID=8YFLogxK
U2 - 10.1063/5.0102969
DO - 10.1063/5.0102969
M3 - Conference contribution
AN - SCOPUS:85138222659
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -
