Abstract
Acrylamide inhibits creatine kinase (CK) activities in the brain of rats or mice. However, its effects on genetic expression of CK have not yet been studied. CK mRNA and protein level were examined by RT-PCR and Western blotting, respectively. Neither cytosolic CK (B subunit) and mitochondrial CK (ubiquitous form) mRNA nor B subunit protein was clearly changed in the cerebellum from rats intoxicated with acrylamide (50 mg/kg/day ip for 8 days) and showing clinical neurotoxic signs.
| Original language | English |
|---|---|
| Pages (from-to) | 51-57 |
| Number of pages | 7 |
| Journal | Journal of UOEH |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Mar 2004 |
Keywords
- Acrylamide
- Creatine kinase
- RT-PCR
- Western blotting
- mRNA
