Acrylamide Does Not Cause Apparent Changes in Genetic Expression of Creatine Kinase in Rat Cerebellum

Bambang Wispriyono, Masato Matsuoka, Hideki Igisu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Acrylamide inhibits creatine kinase (CK) activities in the brain of rats or mice. However, its effects on genetic expression of CK have not yet been studied. CK mRNA and protein level were examined by RT-PCR and Western blotting, respectively. Neither cytosolic CK (B subunit) and mitochondrial CK (ubiquitous form) mRNA nor B subunit protein was clearly changed in the cerebellum from rats intoxicated with acrylamide (50 mg/kg/day ip for 8 days) and showing clinical neurotoxic signs.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalJournal of UOEH
Volume26
Issue number1
DOIs
Publication statusPublished - 1 Mar 2004

Keywords

  • Acrylamide
  • Creatine kinase
  • mRNA
  • RT-PCR
  • Western blotting

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