Teeth have been known as the most durable body parts even after the soft tissues and other skeletons are destroyed. Therefore, it has been used to estimate age when several changes after death and traumatic tissue injury occur, or fingerprints can no longer be used due to a particular condition. One of the frequently used methods is histologic methods, including root dentin translucency and tooth cementum annulation. Counting the total of cementum annulation in human teeth and adding it to the average age of tooth eruption will result in estimated age at death. Compared with other morphological methods with higher errors, tooth cementum annulation produces a more accurate method to estimate the age at death. Another dental physiological process often used in estimating adult age is root dentin translucency (RDT). RDT increases with age, starting from the apex and progressing to the coronal of the teeth. Many studies have been conducted to estimate the age at death for adults using tooth cementum annulation and root dentine translucency separately, with conflicting results. However, there were only a few comparisons of these methods available. This review aimed to compare the accuracy of both methods in estimating age for different age groups.