TY - JOUR
T1 - Absence of evidence of Zika virus infection in cord blood and urine from newborns with congenital abnormalities, Indonesia
AU - Putri, Nina Dwi
AU - Dhenni, Rama
AU - Handryastuti, Setyo
AU - Johar, Edison
AU - Ma'roef, Chairin Nisa
AU - Fadhilah, Araniy
AU - Iskandar, Adhi Teguh Perma
AU - Karyanti, Mulya Rahma
AU - Satari, Hindra Irawan
AU - Jumiyanti, Niphidiah
AU - Aprilia, Yuni Yudha
AU - Sriyani, Ida Yus
AU - Dewi, Yora Permata
AU - Yudhaputri, Frilasita A.
AU - Safari, Dodi
AU - Hadinegoro, Sri Rezeki
AU - Rosenberg, Ronald
AU - Powers, Ann M.
AU - Myint, Khin Saw Aye
AU - PRAYITNO, ARI
N1 - Funding Information:
Financial support: This work was supported by the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia, and the U.S. Centers for Disease Control and Prevention. Funding for this work was also provided by the Office of Infectious Diseases, Bureau for Global Health, U.S. Agency for International Development, under the terms of an Interagency Agreement with CDC.
Publisher Copyright:
Copyright © 2020 by The American Society of Tropical Medicine and Hygiene
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.
AB - Zika virus (ZIKV) has recently been confirmed as endemic in Indonesia, but no congenital anomalies (CA) related to ZIKV infection have been reported. We performed molecular and serological testing for ZIKV and other flaviviruses on cord serum and urine samples collected in October 2016 to April 2017 during a prospective, cross-sectional study of neonates in Jakarta, Indonesia. Of a total of 429 neonates, 53 had CA, including 14 with microcephaly. These 53, and 113 neonate controls without evidence of CA, were tested by ZIKV-specific real-time reverse transcription polymerase chain reaction (RT-PCR), pan-flavivirus RT-PCR, anti-ZIKV and anti-DENV IgM ELISA, and plaque reduction neutralization test. There was no evidence of ZIKV infection among neonates in either the CA or non-CA cohorts, except in three cases with low titers of anti-ZIKV neutralizing antibodies. Further routine evaluation throughout Indonesia of pregnant women and their newborns for exposure to ZIKV should be a high priority for determining risk.
UR - http://www.scopus.com/inward/record.url?scp=85082823643&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.19-0593
DO - 10.4269/ajtmh.19-0593
M3 - Article
C2 - 32043460
AN - SCOPUS:85082823643
SN - 0002-9637
VL - 102
SP - 876
EP - 879
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -