TY - JOUR
T1 - A Simple Photometer and Chemometrics Analysis for Quality Control of Sambiloto (Andrographis paniculata) Raw Material
AU - Heryanto, Rudi
AU - Permana, Derry
AU - Tedjo, Aryo
AU - Rohaeti, Eti
PY - 2017/8/29
Y1 - 2017/8/29
N2 - In this paper, we described the use of a light emitting diode (LED)-based photometer and
chemometric analysis for quality control of king of bitter or sambiloto (Andrographis
paniculata) raw material. The quality of medicinal plants was determined by their chemical
composition. The quantities of chemical components in medicinal plants can be assessed
using spectroscopic technique. We used an in-house photometer to generate spectra of
sambiloto. The spectra were analyzed by chemometric methods, i.e. principal component
analysis (PCA) and partial least square discriminant analysis (PLS-DA), with the aim of
herbal quality classification based on the harvesting time. From the results obtained, based
on thin layer chromatography analysis, sambiloto with different collection times (1, 2, and
3 months) contained different amounts of active compounds. Evaluation of sambiloto,
using its spectra and chemometric analysis has successfully differentiated its quality based
on harvesting time. PCA with the first two PC's (PC-1 = 60% and PC-2 = 35%) was able
to differentiate according to the harvesting time of sambiloto. Three models were obtained
by PLS-DA and could be used to predict unknown sample of sambiloto according to the
harvesting time.
AB - In this paper, we described the use of a light emitting diode (LED)-based photometer and
chemometric analysis for quality control of king of bitter or sambiloto (Andrographis
paniculata) raw material. The quality of medicinal plants was determined by their chemical
composition. The quantities of chemical components in medicinal plants can be assessed
using spectroscopic technique. We used an in-house photometer to generate spectra of
sambiloto. The spectra were analyzed by chemometric methods, i.e. principal component
analysis (PCA) and partial least square discriminant analysis (PLS-DA), with the aim of
herbal quality classification based on the harvesting time. From the results obtained, based
on thin layer chromatography analysis, sambiloto with different collection times (1, 2, and
3 months) contained different amounts of active compounds. Evaluation of sambiloto,
using its spectra and chemometric analysis has successfully differentiated its quality based
on harvesting time. PCA with the first two PC's (PC-1 = 60% and PC-2 = 35%) was able
to differentiate according to the harvesting time of sambiloto. Three models were obtained
by PLS-DA and could be used to predict unknown sample of sambiloto according to the
harvesting time.
KW - sambiloto, Andrograpis paniculata, photometer, chemometrics
UR - http://www.jpacr.ub.ac.id/index.php/jpacr/article/view/349/pdf
U2 - 10.21776/ub.jpacr.2017.006.03.349
DO - 10.21776/ub.jpacr.2017.006.03.349
M3 - Article
SN - 2541-0733
VL - 6
SP - 238
EP - 245
JO - The Journal of Pure and Applied Chemistry Research
JF - The Journal of Pure and Applied Chemistry Research
IS - 3
ER -