A simple method in preparing peripheral blood smear rich in mononuclear leucocytes with abundant cytoplasm for micronucleus test

Jeanne Adiwinata, Robertus Tanzil, Retno Wilujeng Susilowa

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This study aimed to develop a simple method to make peripheral blood smear rich in mononuclear leucocytes with abundant cytoplasm. Such blood smear is essential for a simple and inexpensive micronucleus test. Blood samples were obtained from the Indonesian red cross, Central Jakarta. Various methods using combinations of hypotonic treatment and fixation were performed. Nine small scale methods showing good and consistent results were repeated on 10 individuals. Analysis on the ratio of intact cells, cell diameter and cytoplasm width were done on mononuclear cells, Statistical analysis (balanced Anova) were done using a Minitab program for Windows. The best method consists of hypotonic treatment using plasm:aquabidest 4:1,3:1, and2:1, smearing method resulting in 2 slender smears, and slide fixation using methanol: glacial acetic acid 9: 1.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalMedical Journal of Indonesia
Volume7
Issue number4
DOIs
Publication statusPublished - 1 Oct 1998

Keywords

  • Cell diameter
  • Cytoplasm width
  • Fixative
  • Hypotonic treatment

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