A novel model of liver cancer stem cells developed from induced pluripotent stem cells

Said M. Afify; Anna Sanchez Calle; Ghmkin Hassan; Kazuki Kumon; Hend M. Nawara; Maram H. Zahra; Hager M. Mansour; Apriliana Cahya Khayrani; Md Jahangir Alam; Juan Du; Akimasa Seno; Yoshiaki Iwasaki; Masaharu Seno

doi:10.1038/s41416-020-0792-z

A novel model of liver cancer stem cells developed from induced pluripotent stem cells

Said M. Afify, Anna Sanchez Calle, Ghmkin Hassan, Kazuki Kumon, Hend M. Nawara, Maram H. Zahra, Hager M. Mansour, Apriliana Cahya Khayrani, Md Jahangir Alam, Juan Du, Akimasa Seno, Yoshiaki Iwasaki, Masaharu Seno

Faculty of Engineering

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Background: Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown. Methods: In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed. Results: The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. Conclusions: We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.

Original language	English
Pages (from-to)	1378-1390
Number of pages	13
Journal	British Journal of Cancer
Volume	122
Issue number	9
DOIs	https://doi.org/10.1038/s41416-020-0792-z
Publication status	Published - 28 Apr 2020

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title = "A novel model of liver cancer stem cells developed from induced pluripotent stem cells",

abstract = "Background: Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown. Methods: In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed. Results: The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. Conclusions: We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.",

author = "Afify, {Said M.} and {Sanchez Calle}, Anna and Ghmkin Hassan and Kazuki Kumon and Nawara, {Hend M.} and Zahra, {Maram H.} and Mansour, {Hager M.} and Khayrani, {Apriliana Cahya} and Alam, {Md Jahangir} and Juan Du and Akimasa Seno and Yoshiaki Iwasaki and Masaharu Seno",

note = "Funding Information: Funding information This research was supported by the Grant-in-Aid for Scientific Research (A) No. 25242045; Grant-in-Aid for Scientific Research (C) No. 16K07116; the Grant-in-Aid for Challenging Exploratory Research No. 26640079 and Ministry of Education, Culture, Sports, Science, and Technology (MEXT). Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = apr,

day = "28",

doi = "10.1038/s41416-020-0792-z",

language = "English",

volume = "122",

pages = "1378--1390",

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issn = "0007-0920",

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TY - JOUR

T1 - A novel model of liver cancer stem cells developed from induced pluripotent stem cells

AU - Afify, Said M.

AU - Sanchez Calle, Anna

AU - Hassan, Ghmkin

AU - Kumon, Kazuki

AU - Nawara, Hend M.

AU - Zahra, Maram H.

AU - Mansour, Hager M.

AU - Khayrani, Apriliana Cahya

AU - Alam, Md Jahangir

AU - Du, Juan

AU - Seno, Akimasa

AU - Iwasaki, Yoshiaki

AU - Seno, Masaharu

N1 - Funding Information: Funding information This research was supported by the Grant-in-Aid for Scientific Research (A) No. 25242045; Grant-in-Aid for Scientific Research (C) No. 16K07116; the Grant-in-Aid for Challenging Exploratory Research No. 26640079 and Ministry of Education, Culture, Sports, Science, and Technology (MEXT). Publisher Copyright: © 2020, The Author(s).

PY - 2020/4/28

Y1 - 2020/4/28

N2 - Background: Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown. Methods: In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed. Results: The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. Conclusions: We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.

AB - Background: Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown. Methods: In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed. Results: The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. Conclusions: We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.

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JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 9

ER -

A novel model of liver cancer stem cells developed from induced pluripotent stem cells

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