TY - JOUR
T1 - A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
AU - Ahmad, Islamudin
AU - Ambarwati, Neneng
AU - Elya, Berna
AU - Omar, Hanita
AU - Mulia, Kamarza
AU - Yanuar, Arry
AU - Negishi, Osamu
AU - Muńim, Abdul
N1 - Funding Information:
All author is grateful to the Directorate of Research and Humanity Engagement Universitas Indonesia for funding this study via grant “Hibah TADOK 2018” and many thanks to Head Laboratory of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia for providing facilities in our study.
Publisher Copyright:
© 2019 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug.
AB - Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug.
KW - 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene
KW - Angiotensin-converting enzyme inhibitor
KW - Pellucidin A
KW - Peperomia pellucida (L) Kunth
UR - http://www.scopus.com/inward/record.url?scp=85067650740&partnerID=8YFLogxK
U2 - 10.4103/2221-1691.260398
DO - 10.4103/2221-1691.260398
M3 - Article
AN - SCOPUS:85067650740
SN - 2221-1691
VL - 9
SP - 257
EP - 262
JO - Asian Pacific Journal of Tropical Biomedicine
JF - Asian Pacific Journal of Tropical Biomedicine
IS - 6
ER -