A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells

Yogeswari Chandran; Nam Young Kang; Sung Jin Park; Samira Husen Alamudi; Jun Young Kim; Srikanta Sahu; Dongdong Su; Jungyeol Lee; Marc Vendrell; Young Tae Chang

doi:10.1016/j.bmcl.2015.06.037

A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells

Yogeswari Chandran, Nam Young Kang, Sung Jin Park, Samira Husen Alamudi, Jun Young Kim, Srikanta Sahu, Dongdong Su, Jungyeol Lee, Marc Vendrell, Young Tae Chang

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.

Original language	English
Pages (from-to)	4862-4865
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	25
Issue number	21
DOIs	https://doi.org/10.1016/j.bmcl.2015.06.037
Publication status	Published - 1 Nov 2015

Keywords

Fluorescence imaging
Fluorescence-assisted cell sorting
Mouse embryonic stem cells
Single cell PCR
Three germ layers

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10.1016/j.bmcl.2015.06.037

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title = "A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells",

abstract = "Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.",

keywords = "Fluorescence imaging, Fluorescence-assisted cell sorting, Mouse embryonic stem cells, Single cell PCR, Three germ layers",

author = "Yogeswari Chandran and Kang, {Nam Young} and Park, {Sung Jin} and {Husen Alamudi}, Samira and Kim, {Jun Young} and Srikanta Sahu and Dongdong Su and Jungyeol Lee and Marc Vendrell and Chang, {Young Tae}",

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AU - Chandran, Yogeswari

AU - Kang, Nam Young

AU - Park, Sung Jin

AU - Husen Alamudi, Samira

AU - Kim, Jun Young

AU - Sahu, Srikanta

AU - Su, Dongdong

AU - Lee, Jungyeol

AU - Vendrell, Marc

AU - Chang, Young Tae

PY - 2015/11/1

Y1 - 2015/11/1

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AB - Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.

KW - Fluorescence imaging

KW - Fluorescence-assisted cell sorting

KW - Mouse embryonic stem cells

KW - Single cell PCR

KW - Three germ layers

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 21

ER -

A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells

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Keywords

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