A boron-doped diamond electrode decorated with hemoglobin-modified platinum nanoparticles as a biosensor for acrylamide detection

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Abstract

Acrylamide (AA) is a neurotoxin and potential carcinogen. It has been found in various thermally processed foods, e.g., potato chips and biscuits. Thus, simple, rapid, and sensitive methods for AA detection are needed to ensure food safety. Herein, the fabrication of a highly stable AA biosensor is presented. A boron-doped diamond (BDD) was modified by Pt and hemoglobin. In the first step, platinum nanoparticles (Pt NPs) were chemically seeded onto the BDD surface using NaBH as a reducing agent. The electrochemical overgrowth of these Pt NP seeds was conducted at a constant potential of -0.2 V in a 1 mM Pt solution. Then, rapid thermal annealing (RTA) of the BDD/Pt NP composite was conducted at 700 °C under N atmosphere to enhance its stability. After RTA, BDD/Pt NP was electrochemically activated between -0.5 and 1.5 V. Then, further overgrowth was performed using a deposition voltage of -0.2 V to renew the BDD/Pt NP surface. Finally, 0.15-mM hemoglobin was used to modify BDD/Pt NP. The characterization of the resulting surface was performed using scanning electron microscopy. The biosensor exhibited an optimal response (limit of detection = 0.012 nM) at pH 4.9 in a 0.2-M acetate buffer solution.

Original languageEnglish
Article number012011
JournalIOP Conference Series: Materials Science and Engineering
Volume496
Issue number1
DOIs
Publication statusPublished - 22 Feb 2019
Event2nd International Conference on Current Progress in Functional Materials 2017, ISCPFM 2017 - Bali, Indonesia
Duration: 8 Nov 20179 Nov 2017

Keywords

  • acrylamide
  • BDD
  • biosensor
  • hemoglobin
  • platinum

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