Abstract
Derivatization is a modification process to produce a new compound that can be detected using the suitable detector in chromatography analysis. Derivatization reagent is needed to chemically modify a compound. One of a derivatization reagent that is widely used as a fluorogenic and chromogenic reagent in amine group’s analysis using chromatography is 4-Chloro-7-nitrobenzofurazan (NBD-Cl). NBD-Cl is considerable attraction because it plays a role in pre and post-column derivatizations fused in chromatography for increasing the selectivity and sensitivity. This review provides an overview of many papers that have been presented regarding the application of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) in pre and post-column derivatization in amine group’s analysis and its chromatographic condition from the literature between 2000 to 2022.
| Original language | English |
|---|---|
| Pages (from-to) | 20-23 |
| Number of pages | 4 |
| Journal | International Journal of Applied Pharmaceutics |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2023 |
Keywords
- 4-Chloro-7-nitrobenzofurazan
- Amine groups
- Chromatography
- Post column derivatization
- Pre column derivatization