Abstract
Introduction
Embryo quality has always been considered an important predictor of successful implantation and pregnancy. Nevertheless, knowledge of the relative impact of each morphological parameter at the blastocyst stage needs to be increased. Therefore, the aim of this study was to investigate the relationship between blastocyst morphology, aneuploidy and mosaicism.
Methods
This study collected 70 embryos from 25 women who underwent IVF-ICSI cycles in 4 IVF center in Indonesia. Embryos were cultured until blastocyst stage, biopsy was performed on day 5/6 which were the screened for aneuploidy and mosaicism by Next Generation Sequencing (NGS) method. The relationship between blastocyst, aneuploidy and mosaicism were evaluated.
Results
We found that the frequency of euploid embryo were 40% and 60% were abnormal (28.6% aneuploid, 12.8% mosaicism, and 18.4% triploidy). From blastocyst morphology, a grade < 4 blastocyst expansion had significantly higher rate of aneuploidy and mosaicism than the other grade of expansion (p<0.05). Morphology grading for intercellular mass (ICM) and tropectoderm (TE) also showed a significant correlation with aneuploidy (p<0.05) but not for mosaicism. Another finding that maternal age and sperm morphology also affecting aneuploidy, and women who had ≥10 oocyte when oocyte retieval had a higher chance to have aneuploidy (p<0.05).
Conclusions
Blastocyst morphology showed a significant correlation to aneuploidy, but not for mosaicism. However, grading morphology alone can not replace preimplantation genetic testing for aneuploidy. Morphology assesment combined with PGT-A still produce the best results.
Embryo quality has always been considered an important predictor of successful implantation and pregnancy. Nevertheless, knowledge of the relative impact of each morphological parameter at the blastocyst stage needs to be increased. Therefore, the aim of this study was to investigate the relationship between blastocyst morphology, aneuploidy and mosaicism.
Methods
This study collected 70 embryos from 25 women who underwent IVF-ICSI cycles in 4 IVF center in Indonesia. Embryos were cultured until blastocyst stage, biopsy was performed on day 5/6 which were the screened for aneuploidy and mosaicism by Next Generation Sequencing (NGS) method. The relationship between blastocyst, aneuploidy and mosaicism were evaluated.
Results
We found that the frequency of euploid embryo were 40% and 60% were abnormal (28.6% aneuploid, 12.8% mosaicism, and 18.4% triploidy). From blastocyst morphology, a grade < 4 blastocyst expansion had significantly higher rate of aneuploidy and mosaicism than the other grade of expansion (p<0.05). Morphology grading for intercellular mass (ICM) and tropectoderm (TE) also showed a significant correlation with aneuploidy (p<0.05) but not for mosaicism. Another finding that maternal age and sperm morphology also affecting aneuploidy, and women who had ≥10 oocyte when oocyte retieval had a higher chance to have aneuploidy (p<0.05).
Conclusions
Blastocyst morphology showed a significant correlation to aneuploidy, but not for mosaicism. However, grading morphology alone can not replace preimplantation genetic testing for aneuploidy. Morphology assesment combined with PGT-A still produce the best results.
| Original language | English |
|---|---|
| Pages (from-to) | e46 |
| Journal | Reproductive BioMedicine Online |
| Volume | 39 |
| DOIs | |
| Publication status | Published - 1 Aug 2019 |
Keywords
- embryo
- aneuploidy
- mosaicism
- IVF
- NGS