1H32 Development of MSC/PLCL Composite Tube For Cardiovascular Tissue Engineering

Azizah Intan  Pangesty

doi:10.1299/jsmebio.2016.28._1H32-1_

1H32 Development of MSC/PLCL Composite Tube For Cardiovascular Tissue Engineering

Azizah Intan Pangesty

Department of Metallurgical and Material Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The aim of this study is to develop hybrid tubular structure of PLCL porous tube and mesenchymal stem cells (MSCs). Such graft was constructed by layering MSC sheets on porous PLCL tubular graft. Cell proliferation assay confirmed that the number of cells increased steadily during 11 days of in vitro study. Moreover, it was also found that co-culture cell sheet of MSCs and endothelial cells (ECs) exhibited higher proliferation rate than that of MSCs' monoculture cell sheet, indicating that the co-culture cell sheet can modulate higher proliferation of cells. Tensile mechanical testing of the hybrid tubes was also conducted and it was found that the mechanical properties of the hybrid tube with co-culture cell sheets tended to be larger than those of the tube with monoculture sheets.

Original language	English
Title of host publication	The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
DOIs	https://doi.org/10.1299/jsmebio.2016.28._1H32-1_
Publication status	Published - Jul 2016

Keywords

Tissue engineering
Scaffold
Hybrid graft
Cell sheet
Mesenchymal stem cells
Endothelial cells

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10.1299/jsmebio.2016.28._1H32-1_

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title = "1H32 Development of MSC/PLCL Composite Tube For Cardiovascular Tissue Engineering",

abstract = "The aim of this study is to develop hybrid tubular structure of PLCL porous tube and mesenchymal stem cells (MSCs). Such graft was constructed by layering MSC sheets on porous PLCL tubular graft. Cell proliferation assay confirmed that the number of cells increased steadily during 11 days of in vitro study. Moreover, it was also found that co-culture cell sheet of MSCs and endothelial cells (ECs) exhibited higher proliferation rate than that of MSCs' monoculture cell sheet, indicating that the co-culture cell sheet can modulate higher proliferation of cells. Tensile mechanical testing of the hybrid tubes was also conducted and it was found that the mechanical properties of the hybrid tube with co-culture cell sheets tended to be larger than those of the tube with monoculture sheets.",

keywords = "Tissue engineering, Scaffold, Hybrid graft, Cell sheet, Mesenchymal stem cells, Endothelial cells",

author = "Pangesty, {Azizah Intan}",

year = "2016",

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PY - 2016/7

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N2 - The aim of this study is to develop hybrid tubular structure of PLCL porous tube and mesenchymal stem cells (MSCs). Such graft was constructed by layering MSC sheets on porous PLCL tubular graft. Cell proliferation assay confirmed that the number of cells increased steadily during 11 days of in vitro study. Moreover, it was also found that co-culture cell sheet of MSCs and endothelial cells (ECs) exhibited higher proliferation rate than that of MSCs' monoculture cell sheet, indicating that the co-culture cell sheet can modulate higher proliferation of cells. Tensile mechanical testing of the hybrid tubes was also conducted and it was found that the mechanical properties of the hybrid tube with co-culture cell sheets tended to be larger than those of the tube with monoculture sheets.

AB - The aim of this study is to develop hybrid tubular structure of PLCL porous tube and mesenchymal stem cells (MSCs). Such graft was constructed by layering MSC sheets on porous PLCL tubular graft. Cell proliferation assay confirmed that the number of cells increased steadily during 11 days of in vitro study. Moreover, it was also found that co-culture cell sheet of MSCs and endothelial cells (ECs) exhibited higher proliferation rate than that of MSCs' monoculture cell sheet, indicating that the co-culture cell sheet can modulate higher proliferation of cells. Tensile mechanical testing of the hybrid tubes was also conducted and it was found that the mechanical properties of the hybrid tube with co-culture cell sheets tended to be larger than those of the tube with monoculture sheets.

KW - Tissue engineering

KW - Scaffold

KW - Hybrid graft

KW - Cell sheet

KW - Mesenchymal stem cells

KW - Endothelial cells

UR - https://www.jstage.jst.go.jp/article/jsmebio/2016.28/0/2016.28__1H32-1_/_article

U2 - 10.1299/jsmebio.2016.28._1H32-1_

DO - 10.1299/jsmebio.2016.28._1H32-1_

M3 - Conference contribution

BT - The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME

ER -

1H32 Development of MSC/PLCL Composite Tube For Cardiovascular Tissue Engineering

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Keywords

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