TY - JOUR
T1 - +17 C/G Polymorphism In Matrix Metalloproteinase (MMP)-8 Gene And Its Association With Periodontitis
AU - Putri, Hastinefia
AU - Sulijaya, Benso
AU - Hartomo, Bambang Tri
AU - Suhartono, Antonius Winoto
AU - Ibrahim Auerkari, Elza
N1 - Funding Information:
This study was supported by the University of Indonesia, grant number NKB-2334/UN2.RST/ HKP.05.00/2020 (EIA).
Publisher Copyright:
© 2020 Polish Dental Association
PY - 2020/8/30
Y1 - 2020/8/30
N2 - Introduction: Matrix metalloproteinase-8 (MMP-8), encoded by MMP-8 gene (chromosomal location, 11q22.3), is a proteolytic enzyme that is involved in the pathogenesis of periodontitis. Polymorphism of the encoding MMP-8 gene can affect the risk of the disease. Objectives: This study aimed to evaluate the relationship between +17 C/G polymorphism in MMP-8 and periodontitis in an Indonesian sample population. Material and methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the +17 C/G polymorphism in MMP-8 was conducted in stored genomic DNA samples from 100 subjects with periodontitis and 100 healthy controls. Patients were examined in a previous study by a periodontics and diagnosed as periodontitis based on anamnesis and clinical examination (periodontal pocket depth ≥ 4 mm and the presence of clinical attachment loss. Results: The frequencies of the genotypes or alleles in the periodontitis and control groups were not significantly different (genotype, p = 0.283; alleles, p = 0.11). Any remaining trend toward higher frequencies of the polymorphic genotypes or alleles in the periodontitis group was explained by the adjustment for age. Conclusions: The results showed no significant association between the +17 C/G polymorphism in MMP-8 and the risk of periodontitis in the Indonesian population. No change in the significance is expected even with a considerably larger sample.
AB - Introduction: Matrix metalloproteinase-8 (MMP-8), encoded by MMP-8 gene (chromosomal location, 11q22.3), is a proteolytic enzyme that is involved in the pathogenesis of periodontitis. Polymorphism of the encoding MMP-8 gene can affect the risk of the disease. Objectives: This study aimed to evaluate the relationship between +17 C/G polymorphism in MMP-8 and periodontitis in an Indonesian sample population. Material and methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the +17 C/G polymorphism in MMP-8 was conducted in stored genomic DNA samples from 100 subjects with periodontitis and 100 healthy controls. Patients were examined in a previous study by a periodontics and diagnosed as periodontitis based on anamnesis and clinical examination (periodontal pocket depth ≥ 4 mm and the presence of clinical attachment loss. Results: The frequencies of the genotypes or alleles in the periodontitis and control groups were not significantly different (genotype, p = 0.283; alleles, p = 0.11). Any remaining trend toward higher frequencies of the polymorphic genotypes or alleles in the periodontitis group was explained by the adjustment for age. Conclusions: The results showed no significant association between the +17 C/G polymorphism in MMP-8 and the risk of periodontitis in the Indonesian population. No change in the significance is expected even with a considerably larger sample.
KW - +17 C/G polymorphism
KW - Gene polymorphism
KW - MMP-8
KW - Periodontitis
UR - http://www.scopus.com/inward/record.url?scp=85091006992&partnerID=8YFLogxK
U2 - 10.5114/jos.2020.98310
DO - 10.5114/jos.2020.98310
M3 - Article
AN - SCOPUS:85091006992
SN - 0011-4553
VL - 73
SP - 154
EP - 158
JO - Journal of Stomatology
JF - Journal of Stomatology
IS - 4
ER -