@article{4e729cee4e7644a798170296508036e0,
title = "使用外植体和酶促方法从印度尼西亚患者的肿瘤活检中获得乳腺癌的原代培养物",
abstract = "Breast cancer is the most common cause of death in women globally with high rates of heterogeneity. The development of breast cancer treatment is still constrained because of the different responses to its therapy. The use of primary breast cancer culture is invaluable because it provides the same properties as breast cancer itself. Primary culture is used as a tool to determine the proliferative ability of breast cancer cells. However, the use of optimum methods in cultivating primary culture must be evaluated because of the unstable nature of primary culture. In this study, we investigate the optimum method for culturing cells derived from the tumor biopsies of breast cancer patients in Indonesia by comparing explant and enzymatic methods. Breast cancer tissues were obtained from five breast cancer patients, who underwent surgery and incision biopsies. Tissues were further cultured by explant and enzymatic methods. The cell cultures were observed daily using a microscope for up to 30 days. The results showed that the cells cultured using the enzymatic method for more than 16 h were susceptible to microbiome contamination in the following days after enzymatic digestion, while those cultured using the explant method grew well for 30 days. The findings of this research suggested that the explant method gave better results compared with the enzymatic method. The findings of this study provide insight into the optimum conditions for the primary culture of breast cancer cells.",
keywords = "breast cancer, enzymatic culture, explant culture, in vitro, primary culture",
author = "Arum, {Rizki Sekar} and Noor, {Dimas Ramadhian} and Miftahul Husna and Hana Qanita and Abinawanto and Anom Bowolaksono and Julian, {Erwin Danil} and Astari Dwiranti",
note = "Funding Information: Received: June 14, 2022 / Revised: July 12, 2022 / Accepted: August 17, 2022 / Published: September 30, 2022 Fund Project: The Directorate of Research and Development, Universitas Indonesia (The PUTI Q2 Grant No. NKB-1294/UN2.RST/HKP.05.00/2022) About the authors: Rizki Sekar Arum, Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Dimas Ramadhian Noor, Human Cancer Research Center, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Indonesia; Miftahul Husna, Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Hana Qanita, Division of Surgical Oncology, Department of Surgery, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Abinawanto, Anom Bowolaksono, Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Erwin Danil Julian, Division of Surgical Oncology, Department of Surgery, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Astari Dwiranti, Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia Funding Information: We would like to thank the Directorate of Research and Development, Universitas Indonesia, for the financial support through the PUTI Q2 Grant No. NKB-1294/UN2.RST/HKP.05.00/2022. Publisher Copyright: {\textcopyright} 2022 Authors. All rights reserved.",
year = "2022",
month = sep,
doi = "10.55463/issn.1674-2974.49.9.2",
language = "Chinese (Simplified)",
volume = "49",
pages = "11--16",
journal = "Hunan Daxue Xuebao/Journal of Hunan University Natural Sciences",
issn = "1674-2974",
publisher = "Hunan Daxue/Hunan University",
number = "9",
}