Cytation™ 1 Cell Imaging, a tool for imaging application and conventional multi-mode applications. Multi-Mode Reader combines fluorescence and excessive contrast brightfield imaging with conventional multi-mode detection without the expense and complexity normally related to other digital microscopy systems. Cytation 1 Cell Imaging Multi-Mode Reader eliminates the complexities of multi-mode detection without compromising performance. It can be configured with optional fluorescence and high contrast brightfield cellular visualization from 1.25 times up to 60 times magnification without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. BioTek’s unique approach to imaging and microscopy requirements offers a range of cell imaging systems designed to suit many applications from simple, fixed cell assays and slide scanning to advanced, environmentally controlled long term live cell kinetic assays and 3D spheroid formation imaging. Furthermore, automatic XY stage, focus, and LED intensity facilitate imaging throughput, specially for the duration of cell-based totally applications such as three-d spheroid imaging. The multi-mode detection module includes touchy filter-primarily based fluorescence and luminescence in conjunction with monochromator-primarily based UV-Visible absorbance for flexibility in a wide variety of endpoint, kinetic, inject/read, and other assay types. The reader is also equipped with an injector that allows imaging of immediate cell responses upon compound exposure. Cytation1 can be used for cell migration and invasion assays, live cell imaging label-free cell counting, multi-mode reader applications, immunofluorescence, also phenotypic assays.